IMPROVED PHAGE VECTOR SYSTEM IN STREPTOMYCES

Studies on Streptomyces phage. I. Growth characteristics of the Streptomyces griseus host-phage system.

Within recent years, phages capable of infecting various Streptomyces species have been reported (Saudek and Colingsworth, 1947; Reilly et al., 1947; Koerber et al., 1950; Perlman et al., 1951; and Alexander and McCoy, 1956). Hoehn (1949) and subsequently Gilmour and Buthala (1950) demonstrated that streptomyces phage is indigenous to soil, the natural habitat of the host. The streptomyces host...

Isolation and characterization of a phage active against streptomyces griseus

A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

BACKGROUND Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. RESULTS We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails ...

Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2).

The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the put...

Characterization of the replication, transfer, and plasmid/lytic phage cycle of the Streptomyces plasmid-phage pZL12.

We report here the isolation and recombinational cloning of a large plasmid, pZL12, from endophytic Streptomyces sp. 9R-2. pZL12 comprises 90,435 bp, encoding 112 genes, 30 of which are organized in a large operon resembling bacteriophage genes. A replication locus (repA) and a conjugal transfer locus (traA-traC) were identified in pZL12. Surprisingly, the supernatant of a 9R-2 liquid culture c...